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To help sort out those bacteria that have the vgp gene, scientists first attempt to grow the bacteria both in a medium with ampicillin and in a medium without ampicillin. demonstrate your understanding of this step by sorting the four bacterial classes based on where they will grow.

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Bacteria without plasmid will only grow in the media without penicillin since it doesn't have immunity.
Bacteria with recombinant plasmid with VGP gene, non-recombiant plasmid, Recombinant plasmid but no vgp gene can grow in both media(with or without penicillin) because they can withstand penicillin.

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