serum troponin is the most sensitive and specific test for myocardial damage.
In muscle tissue, troponin is located in the groove between actin filaments and is connected to the protein tropomyosin. Tropomyosin obstructs the myosin cross bridge's attachment site in a relaxed muscle, limiting contraction. An action potential causes calcium channels in the sarcoplasmic membrane to open, releasing calcium into the sarcoplasm and causing the muscle cell to contract. A portion of this calcium binds to troponin, changing its shape and revealing myosin binding sites (active sites) on the actin filaments. When myosin binds to actin, a crossbridge forms, signaling the start of muscle contraction.
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