Your review involves analysis of data regarding the effectiveness of the vaccine in clinical trials. One method used to assess vaccine efficacy is indirect ELISA. Arrange the following steps to describe the sequence of events in performing an indirect ELISA. ► View Available Hint(s) Reset Help The patient serum is added to the wells An anti-antibody is added to the wells. An antigen is attached to wells in a plate. The uncoated surfaces of the plate are blocked. Viral antigen-specific antibodies bind to antigen in the wells. Upon addition of substrate, the well changes color. 1st step in ELISA Last step in ELISA

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30-11-2022
Biology

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Your review involves analysis of data regarding the effectiveness of the vaccine in clinical trials. One method used to assess vaccine efficacy is indirect ELISA. Arrange the following steps to describe the sequence of events in performing an indirect ELISA. ► View Available Hint(s) Reset Help The patient serum is added to the wells An anti-antibody is added to the wells. An antigen is attached to wells in a plate. The uncoated surfaces of the plate are blocked. Viral antigen-specific antibodies bind to antigen in the wells. Upon addition of substrate, the well changes color. 1st step in ELISA Last step in ELISA

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dipankarkundu4589 dipankarkundu4589 · Answer 1 · 2022-12-04T07:04:13+01:00

Indirect ELISA uses a two-step detection process where a primary antibody specific for the antigen binds to the target and a labelled secondary antibody against the primary antigen host species binds to the primary antibody to detect the antigen.

Enzyme-linked immunosorbent assay, or ELISA, is a plate-based assay method used to identify and measure soluble compounds like peptides, proteins, antibodies, and hormones. The same technology is also known by different names, such as enzyme immunoassay (EIA). In an ELISA, the target macromolecule (antigen) is immobilised on a solid surface (microplate), where it then forms a complex with an antibody that is connected to a reporter enzyme. The reporter enzyme's activity is measured through incubation with the proper solution to achieve detection.

Learn more about ELISA here

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