When sequencing DNA using an automated system based on the Sanger method, a. Primer for initiation is NOT required in the reactions.
The Sanger sequencing method, also called "chain termination method", is a method of determining the base sequence of DNA. This method is called the Sanger sequence because it was developed in 1977 by his two-time Nobel laureate Frederick Sanger and his colleagues.
DNA sequencing is the process of determining the sequence of the nucleotide bases (As, Ts, Cs, and Gs) of DNA fragments. With the right equipment and materials, sequencing short DNA fragments is relatively straightforward today.
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