irreversible alteration of extracellular vesicle and cell-free messenger rna profiles in human plasma associated with blood processing and storage.

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30-11-2022
Biology

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irreversible alteration of extracellular vesicle and cell-free messenger rna profiles in human plasma associated with blood processing and storage.

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QWCyan QWCyan · Answer 1 · 2022-12-03T19:13:28+01:00

Circulating extracellular vesicles (EVs) and cell-free RNA (cfRNA) are promising biomarkers for early cancer detection.

EVs are a heterogeneous combination of vesicles released from cells2,3,4,5 that are different in size and composition. EVs have cell-specific antigens on their surface that can be antibody tagged for imaging and/or isolation in -omics analyses6 because they are either generated from the plasma membrane or entail multivesicular endosome fusion with the cell surface.

EVs may carry a variety of proteins and nucleic acids, including being a possible carrier of cfRNA, according to mounting evidence9,10. Plasma contains protein-coding mRNA molecules known as cell-free messenger RNAs (cf-mRNA), which may be used as biomarkers11,12. The combination of these analytes for blood processing and storage is of great interest because EVs may transport a variety of extracellular RNAs, including cf-mRNA.

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