Respuesta :
Give the microscopic slide a name. Onto the slide, drop a loopful of bacterial culture. Hot fix and air dry. For various laboratory processes, including the Gram-stain, a smear must be prepared.
Making a smear is done to adhere the bacteria to the slide and stop the sample from slipping away during the staining process. A solid or broth medium can be used to prepare a smear. The steps for preparing a smear for a Gram-stain are listed below.
1. Center a clean slide with one needle of solid bacterial growth or two loops of liquid bacterial growth.
2. If you're working with a solid media, use a water bottle to add just one drop of water to your specimen. Don't include the water if you're using a broth medium.
3. Using your inoculating loop, now thoroughly combine the specimen with the water, and then spread the mixture out to cover around half of the slide's surface area.
4. After putting the slide on a slide warmer, let it dry. Now that the smear is prepared, staining may begin.
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