Flame the loop or needle after each inoculation and before a new inoculation is done because this flame kills any organism that is left on the instrument.
Flame the loop and needle after using it and flame the inoculation loop before and after working with the bacterial culture, because this method will totally remove or or kills the germs and bacteria which is leftover organism that is still on the instrument. Loops can be used for the conversion of liquid media or petri plates.
So we can conclude that Flame the loop or needle after each inoculation and before a new inoculation is done because this flame kills any organism that is left on the instrument.
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