The appearance of colonies of streptococcus pneumoniae is related to the presence or absence of a polysaccharide coat.
Streptococcus pneumonia's extracellular polysaccharide capsule, the most important virulence factor , helps to initiate infection by allowing the bacterium to adhere to host cells and cause inflammation, while also providing protection from the host's immune system. Griffith conducted his experiments with Streptococcus pneumoniae, a bacterium that causes pneumonia. Griffith worked with two strains of this bacterium called rough (R) and smooth (S).Pneumococci produce pneumolysin, which breaks down hemoglobin into a green pigment that can be observed as a large green zone surrounding S. pneumoniae colonies growing on blood-agar plates.
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