In gram staining After the mordant has been added, gram-positive organisms are stained purple-violet and gram-negative organisms are stained violet black.
- Gram-positive cells will be stained a purplish-blue tint at the conclusion of the gram staining process.
- Crystal violet is also taken up by gram negative cells, and much like in gram positive cells, the iodine forms a crystal violet-iodine compound in the cells.
- Gram's Iodine serves as the mordant. This forms a substantial complex that binds to the crystal violet and attaches to the cell membrane.
- In Gram negative bacteria, there is a thin peptidoglycan layer sandwiched between two thin cell membranes.
- The bacteria are first rinsed with crystal violet, a purple stain, then iodine to perform a Gram stain.
- Iodine and crystal violet combine to generate massive complexes that adhere to cells and color them purple.
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