Answer:
double-stranded RNA (dsRNA) homologous to the gene of interest
Explanation:
RNA interference (RNAi) is a naturally occurring mechanism used by eukaryotic cells to suppress gene expression through regulatory non-coding RNAs (e.g., small interfering RNAs, siRNAs) that are complementary to the target gene of interest. These non-coding RNAs can bind to target messenger RNAs (mRNAs) in the cytoplasm in order to trigger mRNA degradation and/or block translation. The RNAi mechanism is widely used in molecular biology laboratories in order to inhibit the expression of target genes and thus understand gene function. The RNAi mechanism can be induced by transfecting into cells long double-stranded RNA (dsRNA) molecules that are complementary to the target mRNA sequence to be degraded. RNAi is initiated by the enzyme Dicer that cleaves dsRNAs into siRNAs (19 to 25 nucleotides), which are then incorporated into an RNA-induced silencing complex (RISC) in order to suppress gene expression at the post-transcriptional level (i.e., the mRNA is degraded after transcription).
In order to use RNA interference (RNAi) to reduce the expression of a gene in an organism, you should inject a double-stranded RNA (dsRNA) hom-ologous to the gene into the organism.
Gene expression can be defined as the transcription of a gene into a messenger ribonucleic acid (mRNA), followed by its subsequent translation into proteins. Thus, gene expression is typically controlled on two (2) main levels and these are:
RNA interference (RNAi) is also referred to as post-transcriptional gene silencing (PTGS) and it can be defined as a biological process in which the expression of a gene in an organism with respect to in which RNA molecules are sequentially reduced (suppressed) by double-stranded RNA (dsRNA), through transcriptional or translational repression.
This ultimately implies that, you should inject a double-stranded RNA (dsRNA) hom-ologous to the gene into the organism, if you wish to use RNA interference (RNAi) to reduce the expression of a gene in an organism.
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