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Your labmate's ligation reaction didn't work. The regular ATP had run out, so he fueled the reaction with dATP from the PCR kit. What advice can you give him

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ebrightosagie

Answer: adding heat to the ligation reaction to provide energy to the system, instead of ATP.

Explanation:

Ligation is referred to as an act of joining, and the term refers in biology to an enzymatic reaction that joins a covalent bond between two biomolecules. The application of DNA ligation in research in molecular biology is defined in this video.

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