10mL volume of 75mM EtOH is necessary for an experiment. What volumes of 1M EtOH and water should be mixed to obtain the working concentration?

Respuesta :

Answer: Volume of the 1M EtOH and water should be 0.75 ml and 9.25 ml respectively to obtain the working concentration.

Explanation:

According to the dilution law,

[tex]C_1V_1=C_2V_2[/tex]

where,

[tex]C_1[/tex] = molarity of stock solution = 1M

[tex]V_1[/tex] = volume of stock solution = ?

[tex]C_2[/tex] = molarity of diluted solution = 0.075 M    (1mM=0.001M)

[tex]V_2[/tex] = volume of diluted solution = 10 ml

Putting in the values we get:

[tex]1M\times V_1=0.075M\times 10ml[/tex]

[tex]V_1=0.75ml[/tex]

Thus 0.75 ml of 1M EtOH is taken and (10-0.75)ml = 9.25 ml of water is added to make the volume 10ml.

Therefore, volume of the 1M EtOH and water should be 0.75 ml and 9.25 ml respectively to obtain the working concentration