Answer:
Resolution in chromatography is a function of "selectivity, number of plates, and capacity factor".
Explanation:
Resolution is a measure of the separation of 2 peaks of different retention time t in a chromatography divided by the combined widths of the elution peaks.
The resolution is denoted as a numerical value, such as 0.3, 4.0, or 1.0
. Resolution not only depend on column, but also on the flow-rate and mobile phase composition, the rate of gradient and the composition and size of peptides themselves.
[tex]R_{s}=\frac{1}{4} \times \frac{\alpha-1}{\alpha} \times \frac{k}{k+1} \times \sqrt{N_{t h}}[/tex]
Where
[tex]R_s[/tex] is the resolution factor.
[tex]\alpha[/tex] is selectivity factor.
K is the retention factor or the capacity factor.
[tex]N_{th}[/tex] theoretical plate number.
