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Indicate whether or not the following antibody combinations could be used to successfully perform your assay. If a pair can’t be used successfully, indicate why not. (6pts)

• Rabbit anti-HA polyclonal antibody as antibody (II) and Goat anti-rabbit polyclonal antibody conjugated to HRP as antibody (III) Circle one: yes no

• Mouse anti-HA monoclonal antibody as antibody (II) and Goat anti-rabbit polyclonal antibody conjugated to HRP as antibody (III) Circle one: yes no

• Mouse anti-HA monoclonal antibody as antibody (II) and Goat anti-mouse polyclonal antibody conjugated to HRP as antibody (III) Circle one: yes no

Respuesta :

palipali964

Answer:

  • Yes
  • No;
  • Yes

Explanation:

  • Yes: The assay would be successful since antibody (III) is raised against rabbit therefore it would recognize and bind to the epitopes on antibody (II) because it is also a rabbit antibody.  
  • No: The assay would unsuccessful since antibody (III) is raised against rabbit and would not bind to the epitopes  on antibody (II)  which is mouse anti-HA.
  • Yes: The assay would be successful and the signal would be amplified since the polyclonal mouse antibody (III) would bind all the epitopes of the monoclonal mouse antibody (II).

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