Answer and Explanation:
A restriction enzyme is a type of endonuclease enzyme that recognizes a specific short nucleotide sequences on the DNA and cuts the DNA only at palindromic sites. There are different types of restriction enzymes:
1. Type I restriction enzyme – It cuts at sites far from the recognition site and requires both ATP and S-adenosyl- L-methionine as cofactor to function.
2. Type II restriction enzymes – It cleaves at short-specific distances from the recognition site and mostly requires magnesium to function.
3. Type III restriction enzymes – It recognizes two separate non-palindromic sites and cleaves at 20–30 base pairs away from a recognition site. It requires both ATP and S-adenosyl-L-methionine .
The restriction enzymes cleave DNA sequences producing either sticky or blunt ends that can be ligated with other sequences.
