1.

What are the minimum requirements for growing cells in culture?

Group of answer choices

Complete culture medium, pH around 7.4 and a 5% CO2 atmosphere

Complete culture medium, pH around 7.4 and a 15% CO2 atmosphere

Complete culture medium, pH around 9.4 and a 5% CO2 atmosphere

Basal medium, pH around 7.4 and a 5% CO2 atmosphere

2.

What is the condition under the air flow hood like

Group of answer choices

Very clean but not absolutely sterile

Absolutely sterile

Complete asepticall

high level of of CO2

3.

How you detach the adherent cell when you need to sub-culture ?

Group of answer choices

All of them are possible method

enzymatic dissociation

Shaking off

using scraper